Bacillus licheniformis has been found to cause infection in several cases of immunocompromised patients. It is a gram-positive, mesophilic bacterium. FIG. Weakly umbonate elevation. Various organisms can grow on solid media. Umbonate elevation. B. licheniformis is a rod-shaped, gram positive motile bacterium. Catalase-positive. The water droplets (exudate) that can be seen on the outer edges of the colony, not in the center, is a result of respiration. These bacteria are commonly known to cause food poisoning and food spoilage. 29. Birds that tend to stay on the ground more than the air (i.e. Without the support of synthetic chemicals, B. subtilis is not the fastest strain it reduces dry-weight plastic by around 1.75% over a term of 30 days. Bacillus Subtilis - The Definitive Guide | Biology Dictionary The bacteria release exoenzymes that hydrolyze the agar. 1 A). Wastewater Bioaugmentation and Biostimulation. As Bacillus subtilis biofilms in worm intestines seem to lengthen the worms lifespan, many human users hope for the same effect. Putative antibiotic resistance genes present in extant Bacillus - PLOS Among the most important species of bacteria that produce medically importan antibiotics are beloging to the genus Bacillus9. What characterizes the colony morphology of Bacillus subtilis? - BYJU'S Picture Source: eurekabrewing.files.wordpress.com. (4) Salkinoja-Salonen S., Vuorio R., Andersson M.A., Kmpfer P., Andersson M.C., Honkanen-Buzalski T., and Scoging A.C. Toxigenic Strains of Bacillus licheniformis Related to Food Poisoning.Appl Environ Microbiol. . Bacillus licheniformis: Opportunistic Pathogen or Probiotic? A circular chromosome is typical of bacteria, mitochondria, and plant chloroplasts. (7) Wecke T, Veith B, Ehrenreich A, Mascher T. Cell envelope stress response in Bacillus licheniformis: integrating comparative genomics, transcriptional profiling, and regulon mining to decipher a complex regulatory network. (4) Ramnani P, Singh R & Gupta R (2005) Keratinolytic potential of Bacillus licheniformis RG1: structural and biochemical mechanism of feather degradation. Microbiol. PDF Isolation and characterization of different strains of Bacillus Gerardi, M. (2016). Bacillus licheniformis was isolated from soil of different house gardens. Independent.ie. Frontiers | Genomic Analysis of Bacillus licheniformis CBA7126 Isolated B. licheniformis, although usually associated with the gut and gastrointestinal tract, can also cause distress in other parts of the body. A white base of hyphae surrounds green and yellow grainy spore formation in the center of the colony. 25. FIG. 40. Bacillus anthracis. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 42: Serratia marcescens morphology (Enlarged view). It can even go as far as causing abortions in pregnancies and impair sperm motility. The Molecular Biology of the Bacilli. FIG. | Privacy Policy, Terms of Use and State Disclosures. Nocardia asteroides cultivated on trypticase soy agar. [6] Colonies tend to be cream-colored, but will turn red in the presence of iron in media, most likely as a result of pulcherrimin. New York, Nova Science Publishers. Optimal growth temperature is 20-37 C. "Seaweed could fight tooth decay scientists". FIG. 9. Sequencing of the 16S rDNA of the isolates followed by BLAST search revealed that nine strains could be identified as<i> Bacillus licheniformis</i> and one . [6][10] There is evidence that red feathers, with psittacofulvin, are more resistant to degradation. Bacillus - Medical Microbiology - NCBI Bookshelf The initial sample was obtained from a swab taken from the bottom of a shoe and incubated at 37 C for 48 hours. J Bacteriol. Bacillus firmus. Hence, a colony of bacteria is a clone of genetically alike bacteria. 23. 39. Rope spores is what causes the spoilage; unfortunately these spores do not get killed during the baking process. (2) Salkinoja-Salonen S., Vuorio R., Andersson M.A., Kmpfer P., Andersson M.C., Honkanen-Buzalski T., and Scoging A.C. Toxigenic Strains of Bacillus licheniformis Related to Food Poisoning.Appl Environ Microbiol. Bacillus megaterium produce a polypeptide capsule - diversity of metabolic types and nutritional requirements (psychrophiles, mesophiles - majority, and thermophiles; as well as alkalophilic, neutrophilic, and acidophilic) - grow well on blood agar medium, frequently are b-haemolytic - some species are producers of antibiotics Endospores Its use as an expression vector for the production of enzymes and . 2006 May. ruled out continue with routine identification major characteristics of bacillus anthracis gram stain morphology large gram positive rods spores may be . (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 6: Lactobacillus plantarum morphology (Enlarged view). B. subtilis uses encompass research and partially-proven inclusion in dietary supplements. Scientists are currently exploring its ability to degrade feathers for agricultural purposes. (1973) The genus Bacillus . This page was last edited on 22 April 2011, at 19:15. websites owned and operated by ASM ("ASM Web Sites") and other sources. All rights reserved. 16. Unknown isolate cultivated on trypticase soy agar. Pulvinate elevation. (Remco Kort, TNO, Netherlands), Figure 27: Bacillus subtilis morphology (Enlarged view). It is sold under the name Alcalase by Novozymes. 2011; 1(3): 120-126. FIG. Endospores allow the organism to survive in a variety of harsh environments (high salt concentrations, for example) until conditions are more favorable, when it returns to a vegetative state. [5][6] It was initially named Clostridium licheniforme by H. Weigmann[7] and renamed Bacillus licheniformis by Frederick D. Ohmic heating has potential uses such as blanching, evaporation and pasteurization of food; it is a high temperature, short time, and a purely bulk heating method. Colonies are described based on size, shape, texture, elevation, pigmentation, and effect on growth medium. They are often beta-hemolytic. Image 12: A petri dish containing mold growth. Iron-oxidizing Thiomonas-like bacteria isolated from mine waters. I realize that the strains causing these problems were wild strains, not strains studied to be probiotics. 44. Abstract. 46. Currently there are many electrical techniques for food processing, one such example is Ohmic heating. Filamentous form, weakly undulate margin. The initial sample was obtained from a swab taken from the bottom of a shoe and incubated at 37 C for 48 hours. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 37: Streptomyces albus morphology (Enlarged view). The ability to turn waste feathers into feed would reduce feed costs and decrease the need for pollutants currently used to degrade these feathers [3]. Statistical analysis with canonical variates gave four well-separated groups, which represented the four species. when cultured together.Bacillus pumilus: colonies on agar smooth and slightly yellowish; can not grow anaerobically; can not hydrolyze starch; can not reduce nitrate. (Tasha L. Sturm, Cabrillo College, Aptos, CA), Figure 50: Serratia marcescens colonial morphology. It can exist in a dormant spore form to resist harsh environments, or in a vegetative state when conditions are good. The isolated colonies were counted and the number of bacteria expressed as Log of Colony Forming Unit (CFU)/g of CSS. one double-stranded DNA molecule contained within a circular chromosome. Although B. licheniformis often occurs in high numbers in these foods, it's presence is not usually regulated in contrast to B. cereus, which is credited with most food poisoning incidents by the Bacillus species. Genome Biol. Circular form, entire margin. The ability of B. licheniformis to form endospores allows it to survive in the harsh environments required to manufacture industrial enzymes, chemicals, and antibiotics. Contamination with this bacterium will make the bread sticky and stringy; the ropy bread will also start to develop a strong odor after contamination. The colony morphology of B. subtilis refers to how it appears in large quantities. By studying the effect of two antimicrobial agents on the vegetative cells and spores of . FIG. B. licheniformis is a motile organism capable of fermenting sugars (lactose, glucose, mannitol) and excreting useful extracellular enzymes including but not limited to: catalase, casease, urease, deaminase, protease, and lipase. Our website services, content, and products are for informational purposes only. This is desired because it is cheap and nutritious. Circular form, entire margin. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 24: Bacillus subtilis morphology (Enlarged view). B. licheniformis is a Gram positive, spore-forming, facultative anaerobic, rod-shaped bacterium. Circular form, umbonate elevation. Bacillus - Logan - Major Reference Works - Wiley Online Library Bacillus licheniformis cultivated on 5% sheep blood agar. This protease is an active ingredient in laundry detergents, removing protein-comprised dirt in clothes. Unknown isolate cultivated on trypticase soy agar. It has a cell wall that is made of a complex. The pigment is opaque white-beige with a mucoid surface over most of the colony. Macrophages are central cells in the immune response . B. subtilis and a few other bacteria types are abel to use polyethylene as their only carbon (energy) source. You can see Bacillus subtilis listed on the label of many probiotics, too. Bacillus is one of the large genera of bacterial strains. milk pasteurization. Partial isolate of an unknown organism on trypticase soy agar exhibiting spreading and an irregular-rhizoid form with lobate margins. II. (1) Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Jorgensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A., Galleron N., Ehrlich S.D., Berka R.M. cultivated on trypticase soy agar. (6)Hughes, R. C. The Cell Wall of Bacillus Licheniformis N.C.T.C. This fungicide can be used on lawns, conifers, tree seedlings, ornamental turf and ornamental plants in outdoor, greenhouse, and nursery sites. Then, using confocal microscopy, we localized the GFP-tagged strain within plant tissues of inoculated grapevine Glera cuttings. The pigment is opaque white-beige with a mucoid surface towards the center of the colony and a matte surface towards the edges. Bacillus licheniformis Gibson 46 | Type strain - BacDive Pseudomonas aeruginosa cultivated on trypticase soy agar. B. licheniformis has proved itself as a multipurpose organism and has gained popularity along with B. subtilis. of B. licheniformis used for the production of a hydrolase enzyme (P87-1511), and two recombinant strains for production of alpha-amylase (P89-1071, and P92-50). The genera Bacillus and Clostridium constitute the family Bacillaceae. Bacillus licheniformis is a bacterium commonly found in the soil. FIG. in LB agar after 24 h of isolation and incubation was large, round, creamy white, with a rough colony surface (Fig. Solved 34. Identify the organism by its colony morphology. | Chegg.com Characteristics of the bacterial colony on agar slants, Characteristic of bacteria in nutrient broth, Microorganisms that can grow on a nutrient agar plate, Factors affecting the colony morphology of bacteria, Shape A bacterial colony can manifest in various, Staphylococcus irregularly arranged in clusters/grape-like. These strains can grow in media with 5-40% NaCl concentrations. Bacillus globigii at 22. : #22892] Gram stain: positive [Ref. Bacillus licheniformis is a bacterium that is commonly found in soil and bird feathers. Bacillus licheniformis, Bacillus subtilis and Bacillus pumilus are some of the strains that are associated with food spoilage and with a wide range of different clinical conditions (Turnbull, 1997). 11. 1965 February; 89(2): 415420. 24. FIG. The desirable properties of this protease are its prevention of clothes shrinkage and fading colors due to its capability to be used at lower temperatures. (D. Barrie Johnson, University of Wales, UK), Figure 32: Thiomonas-like bacteria morphology (Enlarged view). Strain BDBP 071 was successively identified by colony morphology, Gram staining, cell morphology, physio-biochemical tests, and 16S rRNA sequence analysis. PDF INHIBITORY ACTIVITIES OF NISIN AND POTASSIUM SORBATE ON - ResearchGate Isolation of an unknown bacterium on trypticase soy agar (TSA) exhibiting spreading and rhizoid growth. Streptomyces albus cultivated on nutrient agar. : #22892] Colony size: 5 - 8 mm: Information on culture and growth conditions Culture and growth conditions; Temperatures . Irregular form, undulate margin. While current research into intravenous, intranasal, intraperitoneal, and subcutaneous bacteriocin drugs is still in the testing phase, these intracellular antibacterial agents have already arrived on supermarket shelves as B. subtilis-containing probiotic drinks or pills. Weakly irregular form. [13][14], B. licheniformis is used as a probiotic in animal feed, where isolates have been shown to prevent disease and promote growth as well as being commercially available. Bacillus licheniformis is a bacterium commonly found in the soil. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 30: Bacillus licheniformis morphology (Enlarged view). ducks) are common carriers of this bacterium; it is mostly found around the bird's chest area and back plumage. FIG. (4) Salkinoja-Salonen S., Vuorio R., Andersson M.A., Kmpfer P., Andersson M.C., Honkanen-Buzalski T., and Scoging A.C. (6) Veith, B., Herzberg, C., Steckel, S., Feesche, J., Maurer, K. H., Ehrenreich, P., Bumer, S., Henne, A., Liesegang, H., Merkl, R., Ehrenreich, A., Gottschalk, G. (2004). 33. sparrows)and on the water (i.e. The results of this carbohydrate fermentation test read A. AG, AG, K B . 2004;5(10):R77. The probiotic effects of seven newly isolated gut bacteria, from the indegenous honey bees of Saudi Arabia were investigated. Probiotics are considered living microorganisms that help preserve the health of the host who uses them. One isolate matched Bacillus panaciterrae which has not previously been described as a hot-spring isolate. 6346. Endospore formation is actually desired and serves as a great example of prokaryotic development and differentiation. Find common criteria that are used to characterize bacterial growth; Colony shape Size of the bacterial colony Appearance of the colony surface Consistency/Texture Diagnosis was performed according to Gram stain, motility, shape forming, aerobic condition and other tests. Food borne outbreaks usually involve cases of cooked meats and vegetables, raw milk, and industrially produced baby food contaminated with B. licheniformis. The image below shows how Gram-positive bacteria have a much thicker peptidoglycan layer (in purple). FIG. (3) Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Jorgensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A., Galleron N., Ehrlich S.D., Berka R.M. This gives researchers a lot of material to work with in the race to produce a bacteriocide that pathogenic bacteria do not become resistant to. Bacillus licheniformis - microbewiki - Kenyon College Bacillus licheniformis is a common cause. It is a gram-positive, mesophilic bacterium. Microbiology. Can J Microbiol 51: 191 196. Streak plate isolation of Serratia marcescens on trypticase soy agar (TSA) incubated at 37oC for 24 hours then at room temperature for 24 hours to allow for temperature-sensitive pigment production. It was found in the fetal stomach contents showing it was able to enter the bloodstream of the adult and cross the placenta to a bovine fetus. Agriculture Handbook No. B. licheniformis is also an important ingredient in laundry detergent. The effects of temperature(30-40C), fermentation time (24-72 h), initial medium pH (6.0-9.0), carbon sources (soluble, wheat,rice and corn starches) and substrate concentration (5-30 g/L) on the . Microorganism Bacillus licheniformis - microbewiki Bacillus subtilis PspoIIA-gfp viewed at low magnification. Dairy products are at increased risk of being contaminated with toxin-producing isolates of B. licheniformis. There are increased concerns regarding microbial contaminations, from such bacteria as E. coli and B. licheniformis, when it comes to food processing. Lancaster (PA), DEStech Publications. [20], Below is a list of differential techniques and results that can help to identify Bacillus licheniformis from other bacteria and Bacillus species.[27]. There is an increased interest in using a protease isolated from Bacillus licheniformis in laundry detergents. Irregular form, weakly undulate margin. [12] Amylases are also synthesized by B. licheniformis and used for industrial purposes. The complete nucleotide sequence of Bacillus licheniformis consists of the ATCC 14580 genome, which has a circular chromosome of 4,222,336 bp (base pairs) which contains 4,208 predicted protein-coding genes (average size of 873 bp), 7 rRNA operons, and 72 tRNA genes. Feather degrading bacteria may have played an important role in the evolution of molting, and patterns in feather coloration (Gloger's Rule). FIG. could help reduce the prevalence of dental caries. Bacillus amyloliquefaciens is a ubiquitous soil and plant-associated bacterial species which shows structural and adaptative responses to the environment. . Processes | Free Full-Text | Phenol Degradation Performance in Batch ABIS Encyclopedia FIG. Recognition of greater diversity of Bacillus. Bacillus anthracis, the etiological agent of anthrax, is a gram-positive, spore-forming rod, with colonies exhibiting a unique ground-glass appearance, and lacking hemolysis and motility.In addition to these phenotypes, several others traits are characteristic of B. anthracis such as susceptibility to gamma phage, the presence of two virulence plasmids (pX01 and pX02), and specific . Colonies have a sticky consistency when manipulated with a loop. Death kinetics, in this case, involves the intensity of heat treatments and their correlation with the rate of death of a bacterium. Bacillus subtilis PabrB-gfp after 14 days viewed at high magnification. J Bacteriol. Identification and characterization of clinical Bacillus spp. isolates High expression of Bacillus licheniformis alpha-amylase with a Bacillus secretion vector. From MicrobeWiki, the student-edited microbiology resource, Cell Structure, Metabolism and Life Cycle, Manufacture of Enzymes, Chemicals, Antibiotics. A bacterial colony is limited by. (4). FIG. Its optimal temperature for enzyme secretion is 37C. Lab tests have confirmed the enzyme's ability to break up and remove bacteria present in plaque, and thus prevent the build up of plaque. (Richard A. Robison, Gable Moffitt, Neal Thomson, and Marissa Cohen, Brigham Young University, Provo, UT), Figure 45: Streptomyces albus morphology (Enlarged view). Both require the presence of a zinc cofactor to be effective. "Bacillus Subtilis." (Tasha Sturm, Cabrillo College, Aptos, CA), Figure 57: An unknown airborne environmental isolate on Mueller-Hinton agar. This is typical of S. pneumoniae grown anaerobically. 1999 October; 65(10): 46374645. A colony of Bacillus subtilis survived on the outside of a NASA satellite for six years. 51. (Richard A. Robison, Gable Moffitt, Neal Thomson, and Marissa Cohen, Brigham Young University, Provo, UT), Figure 8: Streptococcus pneumoniae morphology (Enlarged view). [3] A small antisense RNA against Subtilisin Carlsberg named BLi_r0872 was discovered in an RNA-seq based study. Isolated from soil, water, feces, urine, blood, sputum,wounds, pleural fluid, nematodes, and insects. Circular form, entire margin. (Tasha Sturm and Marina Sturm, Cabrillo College, Aptos, CA), Figure 55: Isolation of an Unknown Bacterium on Trypticase Soy Agar. The surface of B. licheniformis colonies are often rough and wrinkled, with "licheniform", or hair-like growths. Biologydictionary.net Editors. Appl Environ Microbiol. Tratat de Microbiologie Clinica, Editura Medicala, Bucuresti. White hyphal growth on the outer edges of the colony followed by: concentric rings of green grainy spore formation with exudate (water droplets resulting from respiration); white grainy spore formation; and finally a pinkish center. Specific strains are also used to produce peptide antibiotics like bacitracin and proticin, as well as some specialty chemicals, including citric acid, inosine, inosinic acid, and poly--glutamic acid [1]. Colony morphology of an organism is necessary for its identification. Volume I: Bacillus subtilis. The aim of this study was the isolation and characterization of thermophilic bacteria from hot springs in Jordan. 56. Bacitracin is composed of a mixture of the cyclic polypeptides that B. licheniformis produces; ironically the purpose of Bacitracin is to inhibit the growth of B. licheniformis. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 18: Kurthia morphology (Enlarged view). It transfers to the gastrointestinal tracts of animals and humans via the soil. (Tasha Sturm and Marina Sturm, Cabrillo College, Aptos, CA), Figure 52: Unknown Fungal Isolate on Trypticase Soy Agar Exhibiting Small Round Colonies. 6346. Bacillus megaterium at 37. Epub 2004 Sep 13. Unknown isolate cultivated on trypticase soy agar. Bacillus subtilis is a Gram-positive, rod-shaped bacterium that is the type species of the genus Bacillus, commonly used as a model organism to describe the structure of different species of the genus. 54. [13][15][16] Some isolates have also been found to be probiotic in humans (and are also commercially available),[13][17] but it's been mentioned that clinical trials have not been performed on many of them yet. PDF Attachment I-- Bacillus Licheniformis I. Introduction - Us Epa Image 11: A colony of yeast on the agar plate. Eight strains each of Bacillus subtilis, Bacillus pumilus, Bacillus licheniformis, and Bacillus amyloliquefaciens were analyzed by using pyrolysis gas-liquid chromatography. A pathway that leads to endospore formation is initiated when the bacterium is starved. Gram-positive, straight or slightly curved rods, 1.5-3.0 x 0.6-0.8 m in diameter, Aerobic, facultatively anaerobic. FIG. Subtilosin has proven antimicrobial activity against Gram-negative and Gram-positive bacteria as well as anaerobic and aerobic microorganisms. Isolation and characterization of a Bacillus licheniformis strain A. Proteases are often included in detergents, and amylases in the desizing of textiles and sizing of papers. B. licheniformis can also give more information about the evolution of molting and patterns of color in birds due to its feather degrading capability. Notice thetypical ground glass appearance. It is a visible mass of microorganism that originates from a single mother cell. Bacillus Licheniformis - an overview | ScienceDirect Topics around the bird's chest area and back plumage. New York, Academic Press. 1. Isolation of Low-Molecular-Weight Fragments from the Soluble Mucopeptide. Biochemical Journal 106.1 1968. (3) Rey M.W., Ramaiya P., Nelson B.A., Brody-Karpin S.D., Zaretsky E.J., Tang M., Lopez de Leon A., Xiang H., Gusti V., Clausen I.G., Olsen P.B., Rasmussen M.D., Andersen J.T., Jorgensen P.L., Larsen T.S., Sorokin A., Bolotin A., Lapidus A., Galleron N., Ehrlich S.D., Berka R.M. (Bryan MacDonald, Christopher Adams, and Kyle Smith, Brigham Young University, Provo, UT), Figure 17: Mycobacterium smegmatis morphology (Enlarged view). FIG. MACROSCOPIC APPEARANCE Typical Bacillus spp. The cells often occur in pairs and chains, where the cells are joined together by polysaccharides on the cell walls. [2] Subtilisin Carlsberg (P00780) secreted by B. licheniformis is used as a detergent protease. B. pumilus contains one circular chromosome Each chromosomes having 4000 genes and 3600-3900 proteins . Bacteriocins from B. subtilis include the lanthionine-containing peptide antibiotic (lantibiotic peptide) called subtilin and an antibiotic called subtilosin. B. subtilis is most commonly singular in arrangement. (7) Wecke T, Veith B, Ehrenreich A, Mascher T. http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=genomeprj&cmd=Retrieve&dopt=Overview&list_uids=13082, http://www.epa.gov/pesticides/biopesticides/ingredients/tech_docs/brad_006492.pdf, https://microbewiki.kenyon.edu/index.php?title=Bacillus_licheniformis&oldid=61404. (9). confidence and trust with respect to the information we collect from you on Bacteria groups can be categorized according to specific arrangements. Circular form, entire margin. (5), Bacillus licheniformis is a spore-forming soil organism that contributes to nutrient cycling and has antifungal activity. "Bacillus Subtilis. Colonies are round to irregular in shape, 2-4 mm in diameter, with margins varying from undulate to fimbriate. Nonmucoid strain of Streptococcus pneumoniae cultivated on sheep blood agar. Kurthia sp. Researchers are trying to turn bird feathers into a nutritious livestock feed by fermenting non-digestable proteins on bird feathers with B. licheniformis. Bacillus licheniformis is a bacterium that is commonly found in soil and bird feathers. FIG. Bacillus licheniformis 16S rDNA [1] A bacterial rod is a symmetrical cylinder with rounded ends. Most do not grow well on enteric agars. Streptococcus pneumoniae cultivated on 5% sheep blood agar and showing beta-hemolysis. 19. B. licheniformis is naturally competent for genetic transformation. document.getElementById( "ak_js_1" ).setAttribute( "value", ( new Date() ).getTime() ); 2022 LaboratoryInfo.com. sparrows)and on the water (i.e. FIG. Microorganisms such as bacteria grow on solid media in the form of a colony. Colony Morphology of Various Bacteria - Laboratoryinfo.com Note the rough and dry texture characteristic of this organism's growth. The sample was obtained from a floor swab and incubated at 37C for 48 hours. 427, U.S.D.A., Washington D.C. Buchanan R.E., Gibbons N.E., Cowan S.T., Holt J.G., Liston J., Murray R.G.E., Niven C.F., Ravin A.W., Stanier R.W. General characteristics: Gram-positive, long, spore-forming rods with a diameter of < 0.9 m.