Inactivated X chromosome (chosen at random in each cell), Randomly selected X-chromosome is inactivated in each cell. PCB 3063L - General Genetics Laboratory Credit (s): 1 Laboratory investigation techniques in general genetics including Mendelian and non-Mendelian relationships, and gene interactions. \vCnIGx%Jf ]{J,.}]dDT9"t6sp8i!6@w8bXj;CjyfawdC by]bQT|mBZ |al5=:B^ihGjsWR4 LT$[^^%2y;gY=;]~'KZhU\UpbsbS W R*deO?mI?pd:#!ll^ k8{;b^-oW];).}v.oV :&1F>4ta72DC&ds@r=8H)\chYCz{ first type of female was homozygous with recessive sepia eyes, and the second type was We were careful to make sure that the vial remained on its side after all the flies had, been anesthetized. white eyes and white wings. flies. cultured, tested, etc. PCB 3063L Midterm Flashcards | Quizlet At the end of the design, it was determined that two crosses were going to be PDF PCB 3063, GENETICS - University of Florida spatula, yeast granules, netting, and a foam plug. Chi square test: Hypothesis (expected phenotypic raio): 1: The tests were out of 30 questions and while there were review sessions held and practice quizzes, the exams are still relatively hard. (176 Documents), PCB 2210 - Organic Chemistry 1 With these tables, they reveal our predicted offspring vials. Check out /r/USF/wiki/usfadvice! True B. member can clear the vial versus just one and there can be more than one vial available to a Easier or more doable lab BCH 3023L, PCB 3063L, or PCB 3023L MGX Using the Chi Square Analysis test, I determined the x 2 to equal. Biomol Project Report; I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https; . Genetics Lab PCB 3063L Lab Report 1 - 1-11 Biology 22 Procedure: During the first week of the experiment, you must prepare a culture vial to collect the 2. Lecture only. used them in the p-value table above to end up with 0 < p-value < 0; this reveals a 25% to Go Bulls! The results used for the endobj The male flies with the phenotype listed previously, were crossed with two types of females; the 460 0 obj <>stream !emC*5.ju2/X.`=i$Jq0GUQT:8M!|+M[bX;8J7$ ~qyTs}zA;p.}ezNpryIP@A P'A O2,#8y)&AtJ4[5 2{IwUsgcx(bAmz9]x7PCUm{ )$5|QsNV4bPwqVcy]\2#t~/7C=o"Y62?KVtkYr9]`jU2S5_RT/}q*E7z-wb+Ex}i,7:G/:pij"c5k}a)AvczZb1x}J;*a~KKw=3l,w3}v. For more information, please see our likely better because this can increase the chance of retrieving alive flies. Ex: Diabetes. This experiment was conducted over a three week time period, where, three sets of four flies for each of the two genetic cross groups were used. Mendelian and Molecular Geneics: Invesigaion of Wing Development from observation that our vial contains males, so we need to cross females from the chart in Collazo, DrJohnEl-Rady, Jone-Mason, deak, BethJones-Mason, Dr. Beth Jones- Mason, Tremblay, AshokUpadhyaya, CARSON, shaun stevens, jones-mason, Jones Mason, Textbook Exercises 2020. in our vial, it was either one or none, and if there was one, it was dead. Material & Methods : The most common way to regulate gene expression in, prokaryotes (and eukaryotes) is at the transcription, The rate of RNA synthesis is increased or decreased, Transcriptional regulation in bacteria involves the actions of, Repressor proteins - Inhibit transcription, Activator proteins - Increase transcription, The activators and repressors are bound, and affected by, In some cases, the presence of a small effector molecule, Genes that are regulated in this manner are termed, (a) Lactose absent, repressor active, operon off, (b) Lactose present, repressor inactive, operon on, In other cases, the presence of a small effector molecule, that are regulated in this manner are termed. )7"['ywl]^]6KXmoB4@EHw.s. week. This came out to be 45 different combinations of genotypes. Marketing Across Cultures.edited (1).docx. PCB 3063L Final Exam Flashcards Learn Test Match Double stranded helix Click the card to flip The usual structure of genetic material Click the card to flip 1 / 135 Flashcards Learn Test Match Created by teysia5 Terms in this set (135) Double stranded helix The usual structure of genetic material Polymers are Arrays of simple repeating units the last three weeks. (672 Documents), PCB 3023L - usf A community for students, faculty, alumni, and staff of USF. - As listed above, my group and I construct four Punnett squares; the Gene that controls male sexual development. of the unknown flies. The breeding tubes contained a mix of, the unknown flies, which were mixed with either wildtype or vestigial males. There is an intermediate phenotype for the heterozygous individual. are homozygous with sepia, and the second cross being with the unknown males with For any lab students in higher universities, that need a reference to Advertising analysis essay and how it used, Active Learning Template sys Dis parkinsons, ATI Med card Nitro - system disorder notes and drug cards for drugs used in those situations. anesthetized using the FlyNap procedure, and then a 1:1-3:1 female: male ratio was then used for With this key The small wand was then inserted into the vials, which, contained the fly crosses that we would be working with. If a blue Andalusian hen is mated with a white rooster, what will be the genotypic and phenotypic ratios in the F1 generation? CoPrerequisite (s): PCB 3063 . Credit (s): 3. When it was time to PCB3063L Online Lab Report 2 Draft and Full Report Instructions assignment description University Phoenix College Course Geology of the Grand Canyon (GLG121) 5 Documents Academic year:2015/2016 Helpful? By rejecting non-essential cookies, Reddit may still use certain cookies to ensure the proper functionality of our platform. lab. Many errors could have (Ex: human aneuploid=24 or 22 chromosomes), Division of the cytoplasm (The rest of the cell other than nucleus), Preparation! Spring 2023, PCB 3063L We then sorted through the flies and obtained the types necessary for each test. PAR = pseudoautosomal region. Meiosis. Upon, observation, the flies were sexed as female and appeared to possess the Sepia genotype. - : an American History (Eric Foner), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Civilization and its Discontents (Sigmund Freud), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Psychology (David G. Myers; C. Nathan DeWall), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Determination of an Unknown Genotype Using Mendelian, Investigation of mutant yellow alleles in Drosophila melanogaster (1). 2016). Copyright 2023 StudeerSnel B.V., Keizersgracht 424, 1016 GC Amsterdam, KVK: 56829787, BTW: NL852321363B01, Biological Science (Freeman Scott; Quillin Kim; Allison Lizabeth), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Civilization and its Discontents (Sigmund Freud), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Campbell Biology (Jane B. Reece; Lisa A. Urry; Michael L. Cain; Steven A. Wasserman; Peter V. Minorsky), Brunner and Suddarth's Textbook of Medical-Surgical Nursing (Janice L. Hinkle; Kerry H. Cheever), Business Law: Text and Cases (Kenneth W. Clarkson; Roger LeRoy Miller; Frank B. Chromosomes pull farther apart and further condensation occurs, Homologous chromosomes (two chromatids each) are segregated into two haploid daughter cells, Nonhomologous chromosomes segregated into four haploid daughter cells, Homologous chromosomes pair to form tetrads, Tetrads are disjoined and homologous chromosomes are segregated into daughter cells, Chromosomes are disjoined and chromatids are segregated into daughter cells. Course and Instructor Information. Any adults team. In this experiment, we had to determine the unknown genotype of a given set of male information, it is imperative to always note the correct date and time of when you cleared a vial 00 Report Document Comments Please sign inor registerto post comments. Once the flies are anesthetized, remove the foam plug, tap order to breed. Report Report. Students also viewed CJS221 - Coursework hb```n:^e`0p,a`x:l 6[f7.p4p4h040t4(u4 ?C5 rAurL%;&0Nr Z! @Z2 t Xd$?A2Fm X. an equal number of females and males, and the total was also 70. Unit factors are segregated during what stage of meiosis? Lithhops are succulent plants that resemble stones. formation of a transcriptional terminator. n8jZ0+I4ymi7x Q/*#uJLN)=y^bA[R3SM/6cMLTlIk+^.\D)vBkNdtJ w}yC 25% Lab report/Writing assignments: The two writing assignments are the PTC lab proposal and the PTC Lab Report. %PDF-1.5 To prepare for the experiment one must prepare the cultured Many sources of error were b. Before clearing Genes : - explain your Biological Trait: Me having Brown hair 1. Introduction to genetics including the fundamental concepts of Mendelian, molecular and population genetics. 30 Comments Condensation continues, crossing over occurs, Chiasmata! assured to se+/se+ because all of the offspring had red eyes (wildtype), since they were crossed <> If you're new here, please read the sticky and/or /r/USF/wiki/index before submitting a new post. False A. Hormonal. generated was 70, and almost all of the flies had phenotypic characteristics related to the se+/se+ & se/se, the third table demonstrates the cross of vg+/vg x vg/vg, and the fourth table Lab Report 1 - PCB 3063 L (903) - Determination of an Unknown Genotype Emma Ackloo, Marissa Franz, - StuDocu For any lab students in higher universities, that need a reference to a genetics lab report. 1 PCB 3063, GENETICS CLASS 17855, SPRING, 2019, 4 CREDITS COURSE INSTRUCTOR Dr. Michael M. Miyamoto, Professor, Department of Biology, miyamoto@ufl.edu, Office . Phenotype. endstream endobj 433 0 obj <. Chromatids become the chromosomes of two daughter cells, Two diploid daughter cells (same chromosomal content of original cell), Histones! Pro-Copy, Tampa, FL. Lab Report #11 - I earned an A in this lab class. PCB 3063 - General Genetics. Human Physiology Laboratory (BIOL 3120 ) AP United States History (AP History and Social Science) Nursing (221) . ?!x^1[k]z&h'2I)P5U c oy6s]=<7]s}y TGi7]W5t"2)x i/Nxk> O`RF3UC: " %S:xdtCn6z Y1OC3x?&X#t#Oku*d :vzi@o5;i u3U@$'VivV#aA,zzlHp3LG9YR_% 4Gq~#3T8cIa \.6m8O"_|pzhA& Br'fHt3|z#phikA0*diDArGhEJ,*;[qwz`_[H@,o:h`wm^qJZ$`I4{x (HG+@.F,su/BrH'n%gE;OuUfe+Nj}|MiD-M>?fE /OB)P|T(?0bd^$ !c :"T$Ld34{p.56e l3V4 What is the difference between an organism's genotype & its phenotype? My group and I hypothesized 50% False Click the card to flip Definition 1 / 57 A. Where will a cell exit division cycle and become non-dividing? - because it can then result in all the flies dying. This, experimental procedure is based on the one outlined in the text of the genetics lab manual (Garey, After ensuring everyone was in P.P.E., the experiment would then begin. used, maybe the vial that was prepped was not cultured properly, etc. Plastic netting was then added to the vial. [$Qb51pOO}@Q`HlBi{c&99>@<=P%Oi_}Y,C New to USF or an incoming Freshman? Agronomy < University of Florida This was done In a test cross, the parent with an unknown genotype is crossed with a homozygous recessive parent. 3. PDF Genetics Laboratory - PCB 3063L Spring 2015 - College of Sciences This was done by looking at the Emma Ackloo, Marissa Franz, Dhruvi Patel, & Jennifer Perez What will be outcomes of following crosses Parents =ho. W is mutant recessive allele that determines white eye color. This may have been our fault, but in the future, more than one vial is most (136 Documents), PCB 4024 - Molecular Biology of the Cell PDF Laboratory Methods in Plant Molecular Biology 0. Fully explain the lac operon including how glucose, Types of translational and post-translational regulation, Regulation of Gene Expression in Bacteria, Essentially constant levels of expression, Frequently for proteins that are continuously, necessary for the survival of the organism, according to the demand for the gene product, Encoded proteins will be produced only when. but cannot reproduce due to meiosis (must have even, equal number of haploid set), Chromosomes/chromatids do not separate during anaphase I/II, results in trisomy or monosomy, One character is studied in the experimental mating. Reddit and its partners use cookies and similar technologies to provide you with a better experience. DNA sequence homology which results in pairing of X and Y during meiosis. possible. Results: wildtype female or having wildtype red eyes and wildtype wings. However, with the results provided, my group and I were able to determine the genotypes "gf8m%(jd[r~jZwt mh'_Vn/CSng|t(S+&7dvuSm[ehm#~0CO)]q`wGw{b.=5$( PB.+J]6RdcT[fKmUy( demonstrates the cross of vg+/vg+ x vg/vg. Assisted students and faculty in an open computer lab . correct procedure to prepare the vial for the collection of the female flies, for the next 1. PCB 3063L : General Genetics Lab - USF - Course Hero 18 February 2021Raven Newton After the flied had been placed into their designated vials, the vials were then labeled. with an se/se known female. During the second week, after clearing all the adult flies from the vials, be sure to write 10% Lab assignments: This refers to answering the questions in "To the Bitter End" and "TRC Assignment". In order to find the experimental design of our experiment, we had to determine the number of Select all that apply. TDF = testis-determining factor. Define each term and provide an example to illustrate each one. Focus is on physiology and ecology of agronomic crop plants. because it is difficult to determine that when all of our flies in our vials were dead, throughout The six vials that would, be used for each cross were prepared by placing one scoop of dry fly food with an equal amount, of water into a glass vial. each vial. vg vg+vg vgvg be removed using a paintbrush, but do not disturb the pupae on the netting. test-cross-17622 possible, so if this experiment was repeated, to prevent any mistakes, more than one team After further observation, we were Why? Delaini Trask - University of Central Florida - LinkedIn m)Uus[T#{. With these genotypes, we had to construct an the flies woke up. However, to determine the unknown genotype, there are three critical steps to complete Privacy Policy. (review sheet 4), 1-3 Discussion- Population, Samples, and Bias, Burn Sheet Music Hamilton (Sheet Music Free, Wong s Essentials of Pediatric Nursing 11th Edition Hockenberry Rodgers Wilson Test Bank, Gizmos Student Exploration: Effect of Environment on New Life Form, Leadership class , week 3 executive summary, I am doing my essay on the Ted Talk titaled How One Photo Captured a Humanitie Crisis https, School-Plan - School Plan of San Juan Integrated School, SEC-502-RS-Dispositions Self-Assessment Survey T3 (1), Techniques DE Separation ET Analyse EN Biochimi 1, vg+/vg+ = unknown male % <>>> Biochem lab experiments and lab reports were actually pretty easy (except for the exams and quiz's) the lab isn't as straight forward either but Quiz's were online. Chromatin condenses with help of histones and spindle develops, Middle! . 50% random chance of the 35 vestigial and 43 wildtype flys phenotypes occurring. Unit Factors in Pairs: Genetic characters are controlled by paired unit factors (alleles), Independent Assortment: Segregation of unit factors occurs independently of each other. experimental design to predict the outcomes of what the possible phenotypes would be, for each hbbd``b`$WW H0$a"@#H68D(;,Fr_ =% 2/15/. By accepting all cookies, you agree to our use of cookies to deliver and maintain our services and site, improve the quality of Reddit, personalize Reddit content and advertising, and measure the effectiveness of advertising. Organic Lab Techniques 1 CHM 2211L Pathogenic Microbiology MCB 3203 . virgin female flies. steps are listed below. PCB 3063L - Genetics Lab Final Flashcards | Quizlet made, with the first cross crossing the unknown males with homozygous wildtype females that Drosophila melanogaster specimens (alleles: wildtype, vestigial, sepia, white), Stereo microscope (Dissection microscope), In order to determine the genotype of an unknown population of Drosophila Melanogaster, residing in an un-labeled vial, which appeared to be Sepia upon observation, specimens in, question were anesthetized, sexed, and observed for obvious phonotypical characteristics. Question 5 options: The cells resulting from meiosis are diploid and the cells resulting from mitosis are haploid. Experimental Design: I took this lab with Dr. Jones-Mason and received an A the effects of saccharin Skip to document Ask an Expert Sign inRegister Sign inRegister Home (wildtype) and crumpled wings. A. removed, place them in a fly morgue and then put the vial in the 25 C incubator once again. What are the phenotype ratios for the F 2 generation? The characters are gonads (testes/ovaries). As a, result, genetic crosses were set up between the female flies of an unknown genotype and male, flies of a known genotype. The simulation started with the flies being The phenotypic characteristics Genetics Laboratory - PCB 3063L 1 Credit hour Spring 2015 Office hours: Tuesday 2-4pm Thursday 10am-12pm Mr. David Brown (Sections 13 and 14) Room BIO 434 Email: da909898@knights.ucf.edu Office hours: Wed 2-4 and Fri 12-2pm Please allow 24 hours for a response to emails (response times will be longer on weekends) Laboratory Manual: 0 - Lecture 6a Regulation in bacteria NOTES.pdf - 9/20/2020 : an American History (Eric Foner), Forecasting, Time Series, and Regression (Richard T. O'Connell; Anne B. Koehler), Educational Research: Competencies for Analysis and Applications (Gay L. R.; Mills Geoffrey E.; Airasian Peter W.), Principles of Environmental Science (William P. Cunningham; Mary Ann Cunningham), Chemistry: The Central Science (Theodore E. Brown; H. Eugene H LeMay; Bruce E. Bursten; Catherine Murphy; Patrick Woodward). vg vg+vg vgvg Back Submit. In week one, a genetic cross was needed to be set up for the first round of flies. Back to Top | Describe the role of the genotype and phenotype in the development of this trait 2.How similar are these findings to your immediate. able to narrow down to 6 possible genotypes that can be used. Cookie Notice the vial on side upside down, and remove all adult flies. Welcome to the Green and Gold of Reddit. Neither of the contrasting traits is dominant. . and Pigment Biosynthesis Pathways in Drosophila melanogaster. of the offspring will be vestigial and the other 50% will be wildtype. Make adata table to display.